Optimized Study on PCR Amplification Procedure of the Apple Self-incompatibility Gene 苹果自交不亲和基因PCR扩增程序优化
The amplification procedure of 94 ℃ 3 min, 1 cycle; 适宜的扩增程序为94℃预变性3min,1个循环;
However, their broader application is hampered by the large amount of RNA requirement, so the T7 RNA linear amplification procedure has gradually become the most commonly used protocol for target preparation when doing microarray experiments. 但常规方法需要大量RNA,因而基于T7RNA线性扩增技术逐渐成为微阵列表达谱实验中最常用的探针制备方法。
The method makes the optimizing process of RAPD amplification conditions a procedure and optimizing conditions expressed with quantity. It is a handy rapid economical practical met hod. 该方法使RAPD扩增条件优化过程实现了程序化和数量化,是获得RAPD优化条件的简便快速、经济实用方法。
The polymerase chain reaction ( PCR) is an in vitro DNA amplification procedure. 多聚酶链反应是体外DNA增殖的新技术。
The optimal PCR amplification procedure indues: Pre-denaturation at 94 ℃ for 4 min; 确定了适合的PCR扩增程序为:94℃预变性4min;
The details are summarized as follows: ( 1) A highly sensitive chemiluminescence immunosensing method for the detection of prostate-specific antigen ( PSA) was developed based on a novel amplification procedure with the application of enzyme encapsulated liposome ( Chapter 2). 简述如下:1.基于脂质体的多功能性,提出了一种利用脂质体为信号放大载体的化学发光生物传感技术对前列腺特异性抗原(PSA)的灵敏检测【第2章】。
It satisfies the detection of polygenic locus by the amplification of genome. With the use of random primer, PEP may keep a good balance in amplification procedure. 结论PEP是一种建立在特殊反应条件下的微量全基因组扩增技术,其对模板的放大能够充分满足多基因位点同时检测的需要,随机引物的使用使其扩增具有良好的平衡性。